The SalmoSim system is composed of a series of bioreactors that simulate two major compartments in the salmon gut: stomach and pyloric caecum/mid-intestine. Temperature, pH, dissolved oxygen levels, enzymatic components and gut microbes mimic those of real salmon. The system has undergone validation via comparison to a parallel in vivo feed trials.
The SalmoSim system can be tailored to your needs for enhanced replication, digestibility models and more.
The salmon gut simulation system
Salmon feed and salmon gut biofluids are mixed with seawater
Temperature, pH, and dissolved oxygen levels are controlled within each bioreator to mimic gut compartment conditions
Digested food is dialysed by pumping through semi-permeable tubing and available nutrients are absorbed
The artificial gut contents are transferred from vessel to vessel via peristaltic pumps
Bioreactor vessels are inoculated with microbial samples from the gut of farmed Atlantic salmon
Bile and mucous from salmon is added to the pyloric caecum compartment daily
Bacterial populations and biochemical parameters (proteins, free amino acids, ammonia etc.) are monitored depending on experimental design
Conditions within bioreactors are continuously monitored using probes and the system can be viewed and controlled remotely
While the system is running we use state of the art microbiological techniques, quantitative PCR and next generation technologies to target specific microbes of interest to characterise prevalence within the our simulated gut environment. Additionally we assess the overall bacterial density.
We use molecular assays and chemical techniques to elucidate the processes of meal digestion and nutrient absoprtion.
We track the levels of dissolved proteins using Bicinchoninic Acid assays and we use phthalaldehyde assays and UPLC-MS/MS to analyse free amino acids. We perform volatile free fatty acids analysis with GC-MS. We analyse the levels of dietary nitrogen within insoluble waste using the Kjeldahl method (an official method of AOAC, USEPA, ISO, Pharmacopeias and different European Directives)
Regarding the digestibility of samples can provide predictive values for in-vivo utilisation of nitrogenous compounds of aquafeed meals. We also provide actionable information on breakdown and solubilisation of proteins and the bioavailability of amino acids and volatile fatty acids.
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